A simple, precise, and reproducible RP-HPLC method has been developed for the simultaneous determination of antihypertensive pharmaceutical tablet dosage form containing ramipril, hydrochlorothiazide and telmisartan. RP-HPLC separation of three drugs was achieved on a Chromatopak peerless basic C18 column (250 mm × 4.6 mm, 5 µm) using UV detection at 215 nm. The optimised mobile phase consisted of acetonitrile and 0.01 M phosphate buffer pH 3 adjusted with o-phosphoric acid (2%, v/v) in a proportion of 60:40 v/v. The flow rate was 0.8 ml min-1. The three drugs were satisfactorily resolved with retention time values of 3.56, 4.75 and 9.23 mins for ramipril, hydrochlorothiazide and telmisartan respectively. The method was validated for linearity, accuracy, precision, robustness, and specificity, as per ICH recommended guidelines. The method was found to be linear over concentration ranges of 9-19, 22.5-47.5 and 72-152 µg ml-1 for ramipril, hydrochlorothiazide and telmisartan respectively. Recovery studies indicated more than 99% of recovery for the three drugs. The relative standard deviation values for intra-day and inter-day precision studies were found to be less than 2% for ramipril, hydrochlorothiazide and telmisartan respectively. No chromatographic interference from any tablet excipients was observed. The method proved to be simple and rapid for routine simultaneous estimation of ramipril, hydrochlorothiazide, and telmisartan in the bulk drug and in a tablet formulation.
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